2326 pET30b-Ecoli-MutH: Difference between revisions

From ELN
Jump to navigation Jump to search
Excellgen (talk | contribs)
confirmed
1,723 edits
No edit summary
 
(No difference)

Latest revision as of 14:06, 8 January 2015

Cloning of 2326 pET30b-Ecoli-MutH

[edit]

Purpose: Cloning of2326 pET30b-Ecoli-MutH==

Date: 2015--1-

Notebook:0

Page:

Clone ID:2326

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA

2326-Ecoli-MutH-Nde1-F tacgacatatgtcccaacctcgcccactg

2326-Ecoli-MutH-Xho1-R tgctactcgagctggatcagaaaatgacgggcca



Digest with Nde1 + Xho1


Colony Screening

[edit]

Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone


Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2326_pET30b-Ecoli-MutH&oldid=13815"