2328 pL42-Tth-PolyA-Pol: Difference between revisions
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Latest revision as of 14:07, 8 January 2015
Cloning of 2328 pL42-Tth-PolyA-Pol
[edit]Purpose: Cloning of2328 pL42-Tth-PolyA-Pol==
Date: 2015-1---
Notebook:0
Page:
Clone ID:2328
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from Tth HB27 genomic DNA
1
2328-Tth-PolyA-Pol-Nde1-F atcagcatatggcccaCatggacttcccct
2328-Tth-PolyA-Pol-R ctgggcctcCaggagggcct
2
2328-Tth-PolyA-Pol-F aggccctcctGgaggcccag
2328-Tth-PolyA-Pol-Xho1-R TGGTGctcgagatgggggtgatctggggttc
1+ 2
2328-Tth-PolyA-Pol-Nde1-F atcagcatatggcccaCatggacttcccct
2328-Tth-PolyA-Pol-Xho1-R TGGTGctcgagatgggggtgatctggggttc
Digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_9
Make miniprep, use Nde1 + Xho1 to find positive clone
