2389 pT7A3E-HCMVUL98: Difference between revisions
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Latest revision as of 14:18, 8 January 2015
Cloning of2389 pT7A3E-HCMVUL98
[edit]Purpose: Cloning of2389 pT7A3E-HCMVUL98==
Date: 2014-12----
Notebook:0
Page:
Clone ID:2389
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2344 pD17-98 use one taq
2345-HCMVUL98-Nde1-F tagac cat atgtggggcgtTtcgagtttg
2389-HCMVUL98-s-Xho1-R2 TGGTGctcgagGCGCGCTTGAGCGTGGCCCTG
Nde1-Xho1
Colony Screening
[edit]Date: 2014_11
Make miniprep, use Nde1-Xho1 to find positive clone