2384 pT7A3E-Apyrase: Difference between revisions
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Latest revision as of 14:19, 8 January 2015
Cloning of2384 pT7A3E-Apyrase
[edit]Purpose: Cloning of2384 pT7A3E-Apyrase==
Date: 2014-11----
Notebook:0
Page:
Clone ID:2384
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
join two fragments by pcr
Digest with Nde1-Xho1
Colony Screening
[edit]Date: 2014_11
Make miniprep, use Nde1-Xho1 to find positive clone
Cloning of2384 pT7A3E-Apyrase
[edit]Purpose: Cloning of2384 T7A3E-Apyrase==
Date: 2014-12----
Notebook:0
Page:
Clone ID:2384
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone from 2380 Digest with Nde1-Xho1
Colony Screening
[edit]Date: 2014_11
Make miniprep, use Nde1-Xho1 to find positive clone
