Yidan 2016 9 30

EXP1

Purpose: Comparison of different Taq

Procedure:

Template: Trec combo(150664295)

Primer: 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R

1 ul of 10 uM primers for 25 ul PCR, 1 ul 10 mM dNTP
0.5ul Taq

PCR cycle:

1 min at 94 oC

30 cycles:
30 s at 94 oC
30 s at 55 oC
1 min at 72 oC

5 min at 72 oC

Result:

PCR product is 1kb

1	invitrogen Taq DNA polymerase Lot:1329498 Exp: 03/2008
2	Qiagen Hot start taq
3	Biolabs One Taq Hot Start DNA polymerase Lot:0011101 Exp:01/2013