MS 5 30 2017

Culture 1782 Tth Ligase (8L), induced with IPTG, over night.

Culture 483 Tth Polymerase (200ml), over night.

Prepare Ni Elution Buffer (50mM phosphate buffer, pH7.0, 250mM NaCl, 100mM Arginine, 300mM imidazole, 10% glycerol)

Wash Ni column (bind with 1708 HTN Cre) with Ni washing Buffer, phosphate buffer and Ni Elution buffer.

Prepare SDS-PAGE Gel Stain.

Prepare Buffer A2, SP Dilution Buffer, Ni Elution Buffer.