831 pMiniT3: Difference between revisions

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Latest revision as of 17:23, 14 August 2017

Digest  832 pMiniT-VVTopo with EcoRV, religate and transform NEB Turbo

PCR screening colonies using pMiniT-seq-F and pMiniT-seq-R

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831-pET28-F

ATCGGATCCGAATTCAGGAGGTAAAAACCATGATATCAGATGGCGCTGGGCGCAATG

831-pET28-R
CTGTTAATTAATTATTATCAGATCAGCTGTTTGATGGTGGTTAACGGCGGGA
(TCCCGCCGTTAACCACCATCAAA CAG CTG ATCTGATAATAATTAATTAA cag  )

PCR fragment from pET28b

ligate to pMiniT2.
positive clone: digest with BamH1 + PacI, religate to cut out insert


RTENOTITLE

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