1949 pT7A3-Kfu-sso7d

Cloning of 1949 pT3A3-Kfu-sso7d

Purpose: Cloning of 1949 pT3A3-Kfu-sso7d

Date: 2013-12-4---

Notebook:0

Page:

Clone ID:1949

Experimenters:  shanyi

Results

cloning

PCR

PCR two fragments from Kod 1801 pT7A30-KOD-sso7d
1
Kfu-F1+Kfu-R2
2

Kfu-F3 c+Kfu-R4

PCR 1 fragment from Pfu 1715 pT7A30-Pfu-sso7d

3
Kfu-F2+Kfu-R3

Join three fragments using

Kfu-F1+Kfu-R4

digest with Nde1 + Xho1 and clone into Nde1 + Xho1 digested vector 1847 pT7A30-sso7d-6H

Colony Screening

Date: 2013-12-6

Make miniprep, digest with NdeI+XhoI

send 1949-2 to sequence with T7term primer

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