Oligos for cloning antibody: Difference between revisions
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Latest revision as of 16:21, 1 May 2018
Mouse B Cells or Hybridoma
[edit]Primers VL-for d V(μl)
original
outer-for: ctacagcaggcccagccggccatggcggactacaaaG
L Q Q A Q P A M A D Y K
ctacagcaggcccagccggccatggcggacgcgcgcGa
L Q Q A Q P A M A D A R
MKYLLPTAAAGLLLLAAQPA
ATGAAATACCTATTGCCTACGGCGGCCGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCCgcgcgcGA
M K Y L L P T A A A G L L L L A A Q P A M A A R
original
19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC
VL-forκ1 catggcggactacaaaGACAWTGTTCTCACCCAGTC 2 6
-- 3 TCAATAGTTGAACATAGCGGCCGCA SAAAWTGTKCTCACCCAGTC κ light chains F3
VL-forκ2 catggcggactacaaaGACATCCAGATGACACAGWC 2 6
VL-forκ3 catggcggactacaaaGATRTTGTGATGACCCAGWC 4 6
--9 TCAATAGTTGAACATAGCGGCCGCA GATATCCAGATGACACAGAC κ light chains F9
--13 TCAATAGTTGAACATAGCGGCCGCA GAYATTGTGATGACBCAGKC κ light chains F13
VL-forκ4 catggcggactacaaaGACATTSTGMTGACCCAGTC 4 6
--18 TCAATAGTTGAACATAGCGGCCGCA GACATTRTGATGWCACAGTC κ light chains F18
--16 TCAATAGTTGAACATAGCGGCCGCA GAAAWTGTGYTGACCCAGTC κ light chains F16
--15 TCAATAGTTGAACATAGCGGCCGCA GACATCYTGCTGACYCAGTC κ light chains F15
--17 TCAATAGTTGAACATAGCGGCCGCA GAAACAACTGTGACCCAGTC κ light chains F17
VL-forκ5 catggcggactacaaaGATGTTGTGVTGACCCAAAC 3 6
--12 TCAATAGTTGAACATAGCGGCCGCA GATRTTKTGATGACYCARAC κ light chains F12
GATGTTTTGATGACCCAAACTCCA 2002 Fab VκA
VL-forκ6 catggcggactacaaaGACACAACTGTGACCCAGTC 1 3
--19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC κ light chains F19
VL-forκ7 catggcggactacaaaGAYATTKTGCTCACTCAGTC 4 6
-- 4 TCAATAGTTGAACATAGCGGCCGCA GAWATTGTGCTMACTCAGTC κ light chains F4
VL-forκ8 catggcggactacaaaGATATTGTGATRACCCAGGM 4 6
note: VL-forκ8 was ordered from IDT as catggcggactacaaaGATATTGTGATRACCCAGGC
maybe should be catggcggactacaaaGATATTGTGATRACCCAGGA
-- 14 TCAATAGTTGAACATAGCGGCCGCA GATATTGTGATAACCCAGGA κ light chains F14
VL-forκ8 catggcggactacaaaGATATTGTGATRACCCAGGC 4 6 modified
VL-forκ9 catggcggactacaaaGACATTGTAATGACCCAATC 1 3
-- 10 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTGACCCAATC κ light chains F10
-- 11 TCAATAGTTGAACATAGCGGCCGCA GACATYSTRATGACCCARTC κ light chains F11
GACATTGTGCTCACCCAATCTCC 2002 Fab VκB
VL-forκ10 catggcggactacaaaGACATTGTGATGWCACAGTC 2 6
--6 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGATGACMCAGTC κ light chains F6
GACATTGTGCTRACCCAGTCTTCCA VκC Fab VκB
-- 5 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTRACACAGTC κ light chains F5
VL-forκ11 catggcggactacaaaGATRTCCAGATGAMCCAGTC 4 6
-- 7 TCAATAGTTGAACATAGCGGCCGCA GAYATCMAGATRAMCCAGTC κ light chains F7
--8 TCAATAGTTGAACATAGCGGCCGCA GAYATCCAGATGAYTCAGTC κ light chains F8
VL-forκ12 catggcggactacaaaGATGGAGAAACAACACAGGC 1 3
optional
VL-forλ1 catggcggactacaaaGACGCTGTTGTGACTCAGGA 1 1
VL-forλ2 catggcggactacaaaGACCYTGTGCTCACTCAGTC 2 2
my design
outer-for: TTATTACTCGCGGCCCAGCCGGCCATGGCCgcgcgcGA
VL-forκ1 GGCCATGGCCgcgcgcGACAWTGTTCTCACCCAGTC 2 6
VL-forκ2 GGCCATGGCCgcgcgcGACATCCAGATGACACAGWC 2 6
VL-forκ3 GGCCATGGCCgcgcgcGATRTTGTGATGACCCAGWC 4 6
VL-forκ4 GGCCATGGCCgcgcgcGACATTSTGMTGACCCAGTC 4 6
VL-forκ5 GGCCATGGCCgcgcgcGATGTTGTGVTGACCCAAAC 3 6
VL-forκ6 GGCCATGGCCgcgcgcGACACAACTGTGACCCAGTC 1 3
VL-forκ7 GGCCATGGCCgcgcgcGAYATTKTGCTCACTCAGTC 4 6
VL-forκ8 GGCCATGGCCgcgcgcGATATTGTGATRACCCAGGM 4 6
VL-forκ9 GGCCATGGCCgcgcgcGACATTGTAATGACCCAATC 1 3
VL-forκ10 GGCCATGGCCgcgcgcGACATTGTGATGWCACAGTC 2 6
VL-forκ11 GGCCATGGCCgcgcgcGATRTCCAGATGAMCCAGTC 4 6
VL-forκ12 GGCCATGGCCgcgcgcGATGGAGAAACAACACAGGC 1 3
optional
VL-forλ1 GGCCATGGCCgcgcgcGACGCTGTTGTGACTCAGGA 1 1
VL-forλ2 catggcggacgcgcgcGACCYTGTGCTCACTCAGTC 2 2
Primers VL-rev
5' (Gly4Ser)3-linker | VL 3'
VL-revκ1 ggagccgccgccgcc (agaaccaccaccacc)2 GCGTTTBATTTCCAGCTTGG 3 25.3
ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCGTTTBATTTCCAGCTTGG
(CCAAGCTGGAAATVAAACGC ggtggtggtggttct ggtggtggtggttct ggcggcggcggctcc)
K L E I/L K R G G G G S G G G G S G G G G S
VL-revκ2 ggagccgccgccgcc (agaaccaccaccacc)2 GCGTTTTATTTCCAATTTTG 1 12.7
ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCGTTTTATTTCCAATTTTG
optional
VL-revλ ggagccgccgccgcc (agaaccaccaccacc)2 GCCTAGGACAGTCAMCYTGG 4 2
ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCCTAGGACAGTCAMCYTGG
Primers VH-for
5' (Gly4Ser)2-linker BamHI | VH 3'
VH-for1 ggcggcggcggctccggtggtggt ggatcc GAGGTTCDSCTGCAACAGTY 12 4
G G G G S G G G G S
ggcggcggcggctccggtggtggt ggatcc GAGGTTCDSCTGCAACAGTC modified
-- 30 TTCACGAGTCCAGCCTCAAGCAGT GAGRTCCAGCTGCAACARTC ---VH primer F30
-- 33 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGMAGCTGGTGGAATC ---VH primer F33
VH-for2 ggcggcggcggctccggtggtggt ggatcc CAGGTGCAAMTGMAGSAGTC 8 3
VH-for3 ggcggcggcggctccggtggtggt ggatcc GAVGTGMWGCTGGTGGAGTC 12 4
-- 35 TTCACGAGTCCAGCCTCAAGCAGT GARGTGCAGCTGKTGGAGWC ---VH primer F35
VH-for4 ggcggcggcggctccggtggtggt ggatcc CAGGTTAYTCTGAAAGAGTC 2 2
VH-for5 ggcggcggcggctccggtggtggt ggatcc GAKGTGCAGCTTCAGSAGTC 2 2
--39 TTCACGAGTCCAGCCTCAAGCAGT GAAGTGAARMTTGAGGAGTC -----VH primer F39
--34 TTCACGAGTCCAGCCTCAAGCAGT GAVGTGMWGCTSGTGGAGTC -----VH primer F34
VH-for6 ggcggcggcggctccggtggtggt ggatcc CAGATCCAGTTSGYGCAGTC 4 2
-- 43 TTCACGAGTCCAGCCTCAAGCAGT CAGATCCAGTTSGTRCAGTC -----VH primer F43
---31 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYVAGCTGCAGCAGTC -----VH primer F31
--Q_mVH_F6 CGTGACCACAGGCGTCCACAGC GACACAACTGTGACCCAGTC 2----
VH-for7 ggcggcggcggctccggtggtggt ggatcc CAGRTCCAACTGCAGCAGYC 4 2
VH-for8 ggcggcggcggctccggtggtggt ggatcc GAGGTGMAGCTASTTGAGWC 8 3
--38 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTTCTCRAGTC ---VH primer F38
--37 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGCAGCTTGTTGAGTC ---VH primer F37
--36 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTGATGGAATC ---VH primer F36
VH-for9 ggcggcggcggctccggtggtggt ggatcc GAAGTGAAGMTTGAGGAGTC 2 2
--27 TTCACGAGTCCAGCCTCAAGCAGT GAKRTRCAGCTTMAGGAGTC ---VH primer F27
VH-for10 ggcggcggcggctccggtggtggt ggatcc GATGTGAACCTGGAAGTGTC 1 1
-- 42 TTCACGAGTCCAGCCTCAAGCAGT GATGTGAACCTGGAAGTGTC ---VH primer F42
VH-for11 ggcggcggcggctccggtggtggt ggatcc CAGATKCAGCTTMAGGAGTC 4 2
VH-for12 ggcggcggcggctccggtggtggt ggatcc CAGGCTTATCTGCAGCAGTC 1 1
-- 40 TTCACGAGTCCAGCCTCAAGCAGT CAGGTTACTCWGAAAGWGTCTG ---VH primer F40
--29 TTCACGAGTCCAGCCTCAAGCAGT CAGGTGCAGMTGAAGSAGTC ---VH primer F29
--28 TTCACGAGTCCAGCCTCAAGCAGT GAGKTYCAGCTBCAGCAGTC ---VH primer F28
VH-for13 ggcggcggcggctccggtggtggt ggatcc CAGGTTCACCTACAACAGTC 1 1
--41 TTCACGAGTCCAGCCTCAAGCAGT CAGGTCCAACTGCAGCAGCC ---VH primer F41
--32 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYCARCTGCAGCAGTC ---VH primer F32
VH-for14 ggcggcggcggctccggtggtggt ggatcc CAGGTGCAGCTTGTAGAGAC 1 1
--44 TTCACGAGTCCAGCCTCAAGCAGT GAGGTRCAGCTKGTAGAGAC ---VH primer F44
VH-for15 ggcggcggcggctccggtggtggt ggatcc GARGTGMAGCTGKTGGAGAC 8 3
Primers VH-rev
5' 3'
outer-rev cggagtcaggcccccgag
5' SfiI VH 3'
VH-rev1 cggagtcaggcccccgaggc CGAGGAGACGGTGACMGTGG 2 2
VH-rev2 cggagtcaggcccccgaggc CGCAGAGACAGTGACCAGAG 1 1
____.SfiI____
(CTCTGGTCACTGTCTCTGCGgcctcgggggcctgactccg)
L V T V S A A S G A -
VH-rev3 cggagtcaggcccccgaggc CGAGGAGACTGTGAGASTGG 2 2
==========
2002 Antibody Display Methods and Protocols,
Fab, Mouse
3'primer
LC Cκ1 CATG TCTAGA ACACTCATTCCTGTTGAAGCTCTTG
5' primers
LC
VκA CATG GAGCTC GATGTTTTGATGACCCAAACTCCA
VκB GATC GAGCTC GACATTGTGCTCACCCAATCTCC
VκC CATG GAGCTC GACATTGTGCTRACCCAGTCTTCCA
VκD CATG GAGCTC GACATCCAGATGACNCAGTCTCAA
VκE CATG GAGCTC CAAATTGTTCTCACCCAGTCTCCA
VκF CATG GAGCTC GAAAATGTGCTTCACCCAGTCTCCA
3' primers
HC
Igγ1 AGGCTT ACTAGT TATGCAAGGCTTACAACC
Igγ2A AGGCTT ACTAGT ACAGGGCTTGATTGTGGGCCC
Igγ2B AGGCTT ACTAGT ACAGGGGTTCAGTGTTGAAATGG
5' primers
HC IA TGGAGGCTT CTCGAG GAKGTGCAGCTTCAGGAGTC
IB TGGAGGCTT CTCGAG CAGGTGCAGCTGAAGSAGTC
IIA TGGAGGCTT CTCGAG SAGGTCCAGCTGCARCAGTC
IIB TGGAGGCTT CTCGAG CAGGTCCARCTGCAGCAGYTTGG
IIC TGG AGGCTT CTCGAG GAGGTTCAGCTGCAGCAGTC
IIIA TGGAGGCTT CTCGAG GARGTGAAGCTGGTGGARTCTGG
IIIB TGGAGGCTT CTCGAG GAGGTGAAGCTTCTGGAGTCTGG
IIIC TGGAGGCTT CTCGAG GAAGTGAAGCTTGAGGAGWCTGG
IIIDA TGGAGGCTT CTCGAG GAAGTGCAGCTGGTGGAGTCTGG
IIIDB TGGAGGCTT CTCGAG GAAGTGATGCTGGTGGAGTCTGG
VA TGGAGGCTT CTCGAG GAGGTYCAGCTKCAGCAG
CH1 GCCAAAACGACACCCCCA
====
leader sequence for the κ light chains
GCCACCATGGAGTTTCAGACCCAGGTACTCATGTCCCTGCTGCTCTGCATGTCTGGTGC
M E F Q T Q V L M S L L L C M S G
GGCCGCACCAGACATGCAGAGCAGCAGGGACATGAGTACCTGGGTCTGAAACTCCATGGTGGC
κ light chains F
3 TCAATAGTTGAACATAGCGGCCGCA SAAAWTGTKCTCACCCAGTC
4 TCAATAGTTGAACATAGCGGCCGCA GAWATTGTGCTMACTCAGTC
5 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTRACACAGTC
6 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGATGACMCAGTC
7 TCAATAGTTGAACATAGCGGCCGCA GAYATCMAGATRAMCCAGTC
8 TCAATAGTTGAACATAGCGGCCGCA GAYATCCAGATGAYTCAGTC
9 TCAATAGTTGAACATAGCGGCCGCA GATATCCAGATGACACAGAC
10 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTGACCCAATC
11 TCAATAGTTGAACATAGCGGCCGCA GACATYSTRATGACCCARTC
12 TCAATAGTTGAACATAGCGGCCGCA GATRTTKTGATGACYCARAC
13 TCAATAGTTGAACATAGCGGCCGCA GAYATTGTGATGACBCAGKC
14 TCAATAGTTGAACATAGCGGCCGCA GATATTGTGATAACCCAGGA
15 TCAATAGTTGAACATAGCGGCCGCA GACATCYTGCTGACYCAGTC
16 TCAATAGTTGAACATAGCGGCCGCA GAAAWTGTGYTGACCCAGTC
17 TCAATAGTTGAACATAGCGGCCGCA GAAACAACTGTGACCCAGTC
18 TCAATAGTTGAACATAGCGGCCGCA GACATTRTGATGWCACAGTC
19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC
κ light chains R
20 GGATACAGTTGGTGCAGCATCAGCCC
Primers used for the generation of the joining fragment
21 GGGCTGATGCTGCACCAACTGTA
22 TCAAGTGCAAAGACTCACTTTATTG
23 CAATAAAGTGAGTCTTTGCACTTGATAGTTATTAATAGTAATCAATTACG
24 TAGCAGAACAGGCAGCTTCATGGTGGCCTGGGAGTGGACACCTGTGGAGAG
25 ATGAAGCTGCCTGTTCTGCTAGTG
26 ACTGCTTGAGGCTGGACTCGTGAACAATAGCAGC
Degenerate forward primers used for the amplification of rearranged
heavy chains
27 TTCACGAGTCCAGCCTCAAGCAGT GAKRTRCAGCTTMAGGAGTC
28 TTCACGAGTCCAGCCTCAAGCAGT GAGKTYCAGCTBCAGCAGTC
29 TTCACGAGTCCAGCCTCAAGCAGT CAGGTGCAGMTGAAGSAGTC
30 TTCACGAGTCCAGCCTCAAGCAGT GAGRTCCAGCTGCAACARTC
31 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYVAGCTGCAGCAGTC
32 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYCARCTGCAGCAGTC
33 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGMAGCTGGTGGAATC
34 TTCACGAGTCCAGCCTCAAGCAGT GAVGTGMWGCTSGTGGAGTC
35 TTCACGAGTCCAGCCTCAAGCAGT GARGTGCAGCTGKTGGAGWC
36 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTGATGGAATC
37 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGCAGCTTGTTGAGTC
38 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTTCTCRAGTC
39 TTCACGAGTCCAGCCTCAAGCAGT GAAGTGAARMTTGAGGAGTC
40 TTCACGAGTCCAGCCTCAAGCAGT CAGGTTACTCWGAAAGWGTCTG
41 TTCACGAGTCCAGCCTCAAGCAGT CAGGTCCAACTGCAGCAGCC
42 TTCACGAGTCCAGCCTCAAGCAGT GATGTGAACCTGGAAGTGTC
43 TTCACGAGTCCAGCCTCAAGCAGT CAGATCCAGTTSGTRCAGTC
44 TTCACGAGTCCAGCCTCAAGCAGT GAGGTRCAGCTKGTAGAGAC
Reverse primers used for the amplification of rearranged heavy chains
45 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACCGTGGTCC
(GGACCACGGTCACCGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG)
T T V T V S S A R L D -
46 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACTGTGAGAGTGGTGC
(GCACCACTCTCACAGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG)
T T L T V S S A R L D -
47 CTATTCTAGCTAATCTAGGCGCGCCGCAGAGACAGTGACCAGAGTCC
(GGACTCTGGTCACTGTCTCTGCGGCGCGCCTAGATTAGCTAGAATAG)
T L V T V S A A R L D -
48 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACTGAGGTTC
(GAACCTCAGTCACCGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG)
T S V T V S S A R L D -
======
Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells
VH1L CCATGGACTGGACCTGGA Heavy chain
VH2L CAGATGGACATACTTTGTTCCAC Heavy chain
VH3L CCATGGAGTTTGGGCTGAGC Heavy chain
VH4L CGATGAAACACCTGTGGTTCTT Heavy chain
VH5L ATGGGGTCAACCGCCATCCT Heavy chain
VH6L GATGTCTGTCTCCTTCCTCAT Heavy chain
VH1F CAGGTGCAGCTGGTGCAGTCTG Heavy chain
VH2F GTCTTGTCCCAGGTCAACTTAAGGGAGTCTT Heavy chain
VH3F GAGGTGCAGCTGGTGGAGTCTG Heavy chain
VH4F CAGGTGCAGCTGCAGGAGTCGG Heavy chain
VH5F GAGGTGCAGCTGCTGCAGTCTG Heavy chain
VH6F CTGTCACAGGTACAGCTGCAGCAGTCAG Heavy chain
IGREV CCAACTCTCTTGTCCACCTTGG Heavy chain
L-Vk1/2 ATGAGGGTCCCCGCTCAGCTGCTGG Kappa light chain
L-Vk3 CTCTTCCTCCTGCTACTCTGGCTCCCAG Kappa light chain
L-Vk4 ATTTCTCTGTTGCTCTGGATCTCTG Kappa light chain
Ck 543-566 GTTTCTCGTAGTCTGCTTTGCTCA Kappa light chain
Pan-Vk ATGACCCAGACTCCATCCACCCTG Kappa light chain
Ck 494-516 GTGCTGTCCTTGCTGTCCTGCT Kappa light chain
L-Vλ1 GGTCCTGGGCCCAGTCTGTGCTG Lambda light chain
L-Vλ12 GGTCCTGGGCCCAGTCTGCCCTG Lambda light chain
L-Vλ3 GCTCTGTGACCTCCTATGAGCTG Lambda light chain
L-Vλ4/5 GGTCTCTCTCGCAGCCTGTGCTG Lambda light chain
L-Vλ6 GTTCTTGGGCCAATTTTATGCTG Lambda light chain
L-Vλ7 GGTCCAATTCCCAGGCTGTGGTG Lambda light chain
L-Vλ8 GAGTGGATTCTCAGACTGTGGTG Lambda light chain
Cλ-1 CACCAGTGTGGCCTTGTTGGCTTG Lambda light chain
F-Vλ1 CAGTCTGTGTTGACGCAGCC Lambda light chain
F-Vλ2 CAGTCTGCCCTGACTCAGCC Lambda light chain
F-Vλa3 TCTTATGAGCTGACACAGCCA Lambda light chain
F-Vλa3l TCTTCTGAGCTGACTCAGGACCC Lambda light chain
F-Vλ4ab GAGCTTGTGCTGACTCAATC Lambda light chain
F-Vλ4c CTGCCTGTGCTGACTCAGC Lambda light chain
F-Vλ5/9 CAGCCTGTGCTGACTCAGCC Lambda light chain
F-Vλa6 AATTTTATGCTGACTCAGCCCCACT Lambda light chain
F-Vλ7/8 CAGACTGTGGTGACCCAGGAG Lambda light chain
F-Vλa10 CAGGCAGGGCTGACTCAG Lambda light chain
Cλ-2 GGGTGGGAACAGAGTGACC Lambda light chain
======
HybriFree: a robust and rapid method for
the development of monoclonal antibodies
from different host species
5'-3' sequences of mouse primers designed by ref. [17]
VH forward primer mix proportion in VHF mix
Q_mVH_F1 CGTGACCACAGGCGTCCACAGC GAGGTTCDSCTGCAACAGTY 4
Q_mVH_F2 CGTGACCACAGGCGTCCACAGC CAGGTGCAAMTGMAGSAGTC 3
Q_mVH_F3 CGTGACCACAGGCGTCCACAGC GAVGTGMWGCTGGTGGAGTC 4
Q_mVH_F4 CGTGACCACAGGCGTCCACAGC CAGGTTAYTCTGAAAGAGTC 2
Q_mVH_F5 CGTGACCACAGGCGTCCACAGC GAKGTGCAGCTTCAGSAGTC 2
Q_mVH_F6 CGTGACCACAGGCGTCCACAGC GACACAACTGTGACCCAGTC 2
Q_mVH_F7 CGTGACCACAGGCGTCCACAGC CAGRTCCAACTGCAGCAGYC 2
Q_mVH_F8 CGTGACCACAGGCGTCCACAGC GAGGTGMAGCTASTTGAGWC 3
Q_mVH_F9 CGTGACCACAGGCGTCCACAGC GAAGTGAAGMTTGAGGAGTC 2
Q_mVH_F10 CGTGACCACAGGCGTCCACAGC GATGTGAACCTGGAAGTGTC 1
Q_mVH_F11 CGTGACCACAGGCGTCCACAGC CAGATKCAGCTTMAGGAGTC 2
Q_mVH_F12 CGTGACCACAGGCGTCCACAGC CAGGCTTATCTGCAGCAGTC 1
Q_mVH_F13 CGTGACCACAGGCGTCCACAGC CAGGTTCACCTACAACAGTC 1
Q_mVH_F14 CGTGACCACAGGCGTCCACAGC CAGGTGCAGCTTGTAGAGAC 1
Q_mVH_F15 CGTGACCACAGGCGTCCACAGC GARGTGMAGCTGKTGGAGAC 3
VH reverse primer mix proportion in VHR mix
Q_mVH_R1 CACCACCACTACCGCCTCCTCCTGAGGAGACGGTGACMGTGG 2
Q_mVH_R2 CACCACCACTACCGCCTCCTCCTGCAGAGACAGTGACCAGAG 1
Q_mVH_R3 CACCACCACTACCGCCTCCTCCTGAGGAGACTGTGAGASTGG 2
VLκ forward primer mix proportion in VLκF mix
Q_mVLκ_F1 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACAWTGTTCTCACCCAGTC 2
Q_mVLκ_F2 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATCCAGATGACACAGWC 2
Q_mVLκ_F3 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATRTTGTGATGACCCAGWC 2
Q_mVLκ_F4 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTSTGMTGACCCAGTC 2
Q_mVLκ_F5 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATGTTGTGVTGACCCAAAC 2
Q_mVLκ_F6 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACACAACTGTGACCCAGTC 1
Q_mVLκ_F7 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGAYATTKTGCTCACTCAGTC 2
Q_mVLκ_F8 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATATTGTGATRACCCAGGM 2
Q_mVLκ_F9 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTGTAATGACCCAATC 1
Q_mVLκ_F10 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTGTGATGWCACAGTC 2
Q_mVLκ_F11 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATRTCCAGATGAMCCAGTC 2
Q_mVLκ_F12 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATGGAGAAACAACACAGGC 1
VLκ reverse primer mix proportion in VLκR mix
Q_mVLκ_R1x GGACAGGTGTGGGTCTTGTCAGCCCGTTTBATTTCCAGCTTGG 2
(CCAAGCTGGAAATVAAACGGGCTGACAAGACCCACACCTGTCC)
K L E X K R A D K T H T C
Q_mVLκ_R2x GGACAGGTGTGGGTCTTGTCAGCCCGTTTTATTTCCAATTTTG 1
(CAAAATTGGAAATAAAACGGGCTGACAAGACCCACACCTGTCC)
K L E I K R A D K T H T C
[[Category:Antibodies]]