3237 pCMV5-(Tev)-Avi-6H-BirA: Difference between revisions

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Latest revision as of 12:09, 23 June 2018

Cloning of

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Purpose:  

Date: 2018-6-22

Notebook: 

Page:

Clone ID:3237

Experimenters: Paul 

Results

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PCR

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Date: Date: 6/21/2018

By: Paul

template:

primers:

Gel:

http://www.excellgen.net/eln/images/5/52/3237_3232_3234_PCR.JPG


Cloning

By: Paul

Vector:  

Ligation: 30 min with T4 DNA ligase

Competent cells: NEBTurbo


Colony PCR Screening 

6/23/18




Restriction Digestion

Date: 6/~/2018

By: Paul




Sequencing




Result intepretation/Summary

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Conclusion

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