345 pET30b-Ent: Difference between revisions
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Latest revision as of 10:39, 30 September 2011
Cloning of by Gene Synthesis
Purpose: to clone
Date: 2011-9-26
Notebook: luyingying
Page:0
Clone ID:345
Experimenters: lu yingying ,
Results
All bands show PCR product of Enterokinase
template:enterokinase gene synthesis ,enterokinase1 to enterokinase 22
primerF: enterokinase1 primerR: enterokinase22
band show enterokinase digest result:Nco1 +Xho1
All bands show PET30b digest result:Nco1 +Xho1
bands show 6 different clones digest result:Nco1 +Xho1
Gene Synthesis
Colony Screening