Yijia LI 6 18 2018: Difference between revisions
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Latest revision as of 14:45, 2 July 2018
Date:6/18/2018
[edit]PCR of 994
PCR of 995
Miniprep of 2509
PCR of 3215
Vector digestion
Date:6/20/2018
[edit]Miniprep of 3215_3204
| # | Lane | Vector | Clone # Name | Method | Enzymes | Size |
| 3215 | 1-5 | 3192 | 1-5 | PCR | Nhe1+Kpn1 | 285 |
| 3204 | 6-10 | 1393 | 1-5 | PCR | Nhe1+Kpn1 | 564 |
3204 digestion
Enzymes:Age1+Kpn1
Result:#3,#4,#5, are good
Date:6/22/2018
[edit]1st PCR fo 3218
2nd PCR of 3218
2509subclone
Vector digestion_1000_3206_3197
1st PCR of 3206
2nn PCR of 3206
3197 PCR
Vector digestion&3225 subclone
PCR of 1000