3210 pT7A30-Sumo-Ov-ASP1-BCMA: Difference between revisions
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Latest revision as of 14:47, 13 July 2018
Miniprep of 3122_3210
Date:6/13/2018
[edit]3210 subclone
Miniprep of 3210
Need to do verfication for clone #4
Nde1-EcoR1: 1 kb
BamH1+ Nde1: 363 bp
BamH1 + Xho1; 613 bp
Redo
[edit]Miniprep digestion_3196_3199_3210
Lane 16 is 3224pminiT2 as + control
Date:7/6/2018
[edit]redo
vector digestion test
| # | Lane | Enzymes | Result | Note |
| 1727 | 1-3 | Nde1+Xho1 | transforma to make new miniprep | The vector from miniprep and GS maybe degrade, 3 is original plasmid, its a single band. |
| 1851 | 4-5 | Nde1+HindIII | need enough conc | 4 is 3191 from 1851 as vector |
3210 subclone
Vector digestion of 3210
Vector is 2289 Nde1+EcoR1
miniprep digestion 3210
