3215 pCMV5-MICA-a-Avi-6H-BirA: Difference between revisions
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Latest revision as of 14:59, 25 July 2018
Cloning of 3215 pCMV5-MICA-a-Avi-6H-BirA
[edit]Purpose: Cloning of 3215 pCMV5-MICA-a-Avi-6H-BirA
Date: 06, 20, 2018
Notebook:
Page:
Clone ID: 3215
Experimenters: yijia
Results
[edit]Cloning
[edit]PCR from 3213 pANT7_cGST-MICA
PCR of 3215
Vector digestion
Vector is 3192
3215subclone
Colony screen
[edit]Miniprep of 3215_3204
| # | Lane | Vector | Clone # Name | Method | Enzymes | Size |
| 3215 | 1-5 | 3192 | 1-5 | PCR | Nhe1+Kpn1 | 285 |
| 3204 | 6-10 | 1393 | 1-5 | PCR | Nhe1+Kpn1 | 564 |
Miniprep digestion _3204_3215_941
Vector is 3237
