3225 pT7A3-GST-MICA: Difference between revisions
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Latest revision as of 16:27, 27 July 2018
Cloning of 3225 pT7A3-GST-MICA
[edit]Purpose: Cloning of 3225 pT7A3-GST-MICA
Date: 06, 22, 2018
Notebook: 008840
Page:008956
Clone ID: 3225
Experimenters: yijia
Results
[edit]Cloning
[edit]Vector digestion&3225 subclone
vector is 1851;Nhe1+Xho1
Miniprep of 3225
Enzymes:Nhe1+Xho1
Size:383bp
actually is junk
redo
[edit]miniprep digestion 3225_999_931
| # | Lane | Vector | Enyzmes | Size | note |
| 3225 | 1-4; 5 is + control | 1851 pT7A3-GST | Nhe1+Xho1 | 383bp | |
| 999 | 6-9;10 is +control | 1873 | Nde1+Xho1 | 1182bp | |
| 931 | 11-14;15 is + control | 931-1 | HindIII+Xho1 | failed |
