941 pT7A3-pMBP-T7-Endo-1: Difference between revisions
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Latest revision as of 16:36, 27 July 2018
Cloning of 941 pT7A3-pMBP-T7-Endo-1
[edit]Purpose: Cloning of 941 pT7A3-pMBP-T7-Endo-1
Date: 06, 28, 2018
Notebook:
Page:
Clone ID: 941
Experimenters: yijia
Results
[edit]Cloning
[edit]subclone from 1225
941subclone
vector 947
redo
Colony screen
[edit]Miniprep of 941
| # | Lane | Vector | Clone # Name | Method | Enzymes | Size | Note |
| 941 | 1-5 | 947 | 12-15;16 is+ | subclone | Nde1+Xho1 | 456bp | #2,#4 might be good |
Miniprep digestion _3205_3215_941
redo
Miniprep digestion of 941_3244_998
| # | Lane | Vector | Method | Enzymes | Size | Note |
| 941 | 1-4; 5 is + control | 947 | Subclone | Nde1+Xho1 | 456bp | |
| 3244 | 6-9 | 3242 | PCR cloning | Nde1+HindIII | 739bp | |
| 998 | 10-13 | 1708 | Gibson Assembly | HindIII+Xho1 |
