LATE-PCR

Primer 1: 25 nM
Primer 2: 1000 nM

95°C for 3 min; 
10 cycles of 95°C for 10 sec, 60°C for 10 sec, and 72°C for 20 sec;
70 cycles of 95°C for 10 sec, 55°C for 20 sec. and 72°C for 20 sec.