Yijia LI 7 30 2018: Difference between revisions
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Latest revision as of 14:44, 7 August 2018
Date:7/30/2018
[edit]129 vector digestion
998 PCR
3206 vector digestion
3244 vector digestion
3244 PCR
129 PCR
931-1 subclone
998 Vector1708 digestion
Date:7/31/2018
[edit]3244 PCR screening
3206 vector digestion
129 vector digestion
Miniprep digestion998_129_3244
Miniprep digestion 2_3244_129_998
Date:8/2/2018
[edit]vector digestion of 3206_998
Individual report
[edit]931
[edit]Subclone of 931
Vector:1987pT7A3
Insert:
F1:1174 bp
F2:986 bp
F1:Nde1+HindIII
F2:HindIII+Xho1
Date:5/29/2018
[edit]Subclone of 931
Vector is 1987pT7A3
Miniprep digestion of 984_931
| # | Lane | Enzymes | Size | Note |
| 984 | 2-5 | Xba1+Nhe1 | 957bp | #6,7,,8 seems good |
| 931 | 1,6-8 | HindIII+Xho1 | 986bp | #5 seems good |
Digest with HindIII +Xho1 and Nde1+ HindIII, Nde1 + Xho1
using parental vector as control. Need to have marker
not good
3rd subclone