3206 pEF1-HTLV-LAMP-1: Difference between revisions

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Latest revision as of 17:00, 9 August 2018

Cloning of 3206 pEF1-HTLV-LAMP-1

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Purpose: Cloning of 3206 pEF1-HTLV-LAMP-1

Date: 06, 22, 2018

Notebook: 008840

Page:008956

Clone ID: 3206

Experimenters: yijia

Results

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Cloning

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PCR from 987 pANT7_cGST-LAMP1

Vector digestion_1000_3206_3197

Vector is 3165

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1st PCR of 3206

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2nn PCR of 3206

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 Colony screen

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Miniprep diegstion of 3206

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need to do PCR screening


Redo

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3206 1st PCR

Template:987

Fragment 1: 3206-F1 + 988-R1
Fragment 2: 988-F3 + 3206-R3

5X enhancer,homemade phusion, 5X thermo phusion HF buffer

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3206 2nd PCR

Template:1st PCR above

Fragment 1: 3206-F1 + 3206-R4

Fragment 2: 3206-F2 + 3206-R3

5X enhancer,homemade phusion, 5X thermo phusion HF buffer

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Result intepretation/Summary

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3206 PCR Screening

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Conclusion

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