417-pAAV-iRFP: Difference between revisions

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Latest revision as of 16:02, 3 October 2011

Cloning of pAAV-iRFP

Purpose: to clone  pAAV-iRFP by subclone from 338 pET28b-iRFP to 254 pAAV-MCS

Date: 2011-9-30

Notebook:Lu yingying

Page:

Clone ID:417

Experimenters: lu yingying
Results

338,254,280,152digest result9-29-2011.JPG

digest result: BamH1+Xho1

bands 1,3,5,7,9,11 show IRFP  insert

band 2 shows PAAV vector

Ligation at RT for 2h


digest detect:BamH1 +Xho1