3256 pCMV5-myc-rIgG: Difference between revisions
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Latest revision as of 14:15, 20 August 2018
Cloning of 3256 pCMV5-myc-rIgG
[edit]Purpose: Cloning of 3256 pCMV5-myc-rIgG
Date: 08, 15, 2018
Notebook:
Page:
Clone ID: 3256
Experimenters: yijia
Results
[edit]Cloning
[edit]1st PCR of 3256
F1: PCR from 3167 pCEH0-Myc-VH-mIgG2a
3256-EcoR1-F1 TGTCACGAATTCAGAGGTGGACCTGGTTGAGTCTG
F2: PCR from 1413 pCDNA3.4-Rabbit-IgG Heavy Chain
3256-F2 CACCACAGTGACCGTTTCTTCTGCTAGCGGTACCGGGCAACCTAAGGCTCCATC
2nd PCR of 3256
Template:combine F1+F2 3256-EcoR1-F1 TGTCACGAATTCAGAGGTGGACCTGGTTGAGTCTG
Vector digestion
Vector is 3075
Colony screen
[edit]
Miniprep of 3256
| # | Lane | Enzymes | Length | Note |
| 3256 | 9-12(#1-4) | EcoR1+Not1 | 1380bp | #2 to do sequence |
| vector control | 3075 | EcoR1+Not1 | 685bp |
