Protein Expression 8 17 2018: Difference between revisions
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Latest revision as of 15:44, 20 August 2018
Condition:0.25mM IPTG, RT, O/N
| Lane | # | Name | Type | Pellet | MW(KD) | Note | Expression level |
| 1 | 3122 | pET30b-CRM197 |
S | loose | 63.1 | Y | very low |
| 2 | 3127 | pET28b-qBeta-qBeta-6XHis |
S | loose | 15.6 | Y | very low |
| M | Spetra BR protein standard | 5,10,25,35,40(red),50(green),70(brown),100,140(purple),260 |
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| 3 | 984 | pT7A3-6H-SBP-SUMO-3C |
S | loose | 27.7 | N | |
| 4 | 3248 | pQE-80L-T7-RNAP-P266L |
S | loose | 99.7 | Y | high |
| 5 | 129 | pT7A30-TthMuts |
S | loose | 91.8 | N | |
| 6 | 3122 | IB | N | ||||
| 7 | 3127 | IB | Y | ||||
| 8 | 984 | IB | N | ||||
| 9 | 3248 | IB | N | ||||
| 10 | 129 | IB | N | ||||
| 11 | 1708 | Cre | S | From protein purification | 43 | Y |