Protein QC 4 23 18: Difference between revisions
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Latest revision as of 16:15, 20 August 2018
Condition:1mM IPTG, 4oC,O/N
| Lane | Sample | Name | MW | Note |
| M1 | SeeBlue | 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191 |
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| 1 | 993(S) | pCold-GST-M13-Gene8 |
31.3 | y? |
| 2 | 992(S) | pCold-GST-V5 |
30.9 | y? |
| 3 | 991(S) | pCold-GST-Flag |
30.5 | Y |
| 4 | 990(S) | pCold-GST-HA |
30.6 | Y |
| 5 | 989(S) | pCold-GST-Myc |
30.7 | Y |
| M2 | homemade protein standard | |||
| 6 | 993(IB) | |||
| 7 | 992(IB) | |||
| 8 | 991(IB) | |||
| 9 | 990(IB) | |||
| 10 | 989(IB) |
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| Lane | Sample | Name | Type | MW | Note |
| M1 | seeblue | 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191 |
|||
| 1 | 947 | pT7A30-6His-pMBP |
S | 46.6 | Y |
| 2 | 986 | pT7A3-6His-NLS-Cas9-NLS-6xHis |
S | 164 | Y |
| 3 | 945 | pT7A30-pMBP |
S | 48.3 | Y |
| M2 | |||||
| 4 | 947 | IB | |||
| 5 | 986 | IB | |||
| 6 | 945 | IB |