136 1708 212 pL6H-Tat-Cre: Difference between revisions
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Latest revision as of 15:28, 9 October 2018
Subcloning:
For 134: vector 212 and insert 1707: Nco1 + Xho1, wrong, should use Nde1 + Not1
For 136: Vector 2038 and insert 129, Nco1 - Xho1
Miniprep digestion
Redo
10/7/2018
| Lane | ID | Name | Enzyme |
| 1 | 129 | Nde1-Not1 | |
| 2 | 2038 | Nde1-Not1 | |
| 3 | 1708 | Nco1-Xho1 | |
| 4 | 212 | Nco1-Xho1 | |
| 5 | 3257 | EcoR1-Nhe1 | |
| 6 | 3260 | EcoR1-Nhe1 | |
| 7 | 3261 | EcoR1-Nhe1 | |
| 8 | 3259 | EcoR1-Nhe1 | |
| 9 | 3258 | EcoR1-Nhe1 | |
| 10 | 3262 | EcoR1-Nhe1 |
