Protein QC 8 10 2018

Experiment condition:

15ml meduim, 0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8.

Prptein Screening of 1000_942(+)_938_939_941

Lane	ID	Name	Type	MW	Expression	Note	Pellet Type
M		SeeBlue Protein 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191
1	1000	pT7A3-6His-NLS-FnCpf1-NLS-6xHis	unbroken cell pellet	157.1	Y		loose
2	942(+)	pT7A3-pMBP-VVN	unbroken cell pellet	24.8	?		tight
3	938	pT7A30-PNGase F	unbroken cell pellet	40.1	?		loose
4	939	pET30b-Tte-ExoIII	unbroken cell pellet	31.3	y		loose
5	941	pT7A3-pMBP-T7-Endo-1	unbroken cell pellet	63.4	N		loose
6	1000		S		Y
7	942(+)		S		?
8	938		S		?
9	939		S		Y
10	941		S		?
11	941 in invitrogen Sample buffer		S

Protein Screening of 3197_994_995_3225_3210

Lane	ID	Name	Type	MW	PI	Expression	Note
M		SeeBlue Protein ladder 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191
1	3197	pT7A3-GST-BCMA	unbroken cell pellet	35
2	994	pT7A3-GST-CD133-EC2	unbroken cell pellet	53
3	995	pT7A3-GST-CD133-EC3	unbroken cell pellet	50.1
4	3225	pT7A3-GST-MICA	unbroken cell pellet	42.5
5	3210	pT7A30-Sumo-Ov-ASP1-BCMA	unbroken cell pellet	36.7
M	SeeBlue Protein Ladder
6	3197		S			Y
7	994		S			Y
8	995		S			?
9	3225		S			Y
10	3210		S			Y

pT7A3-GST-BCMA