Protein QC 8 14 2018

Experiment condition:

M1:Seeblue protein ladder

M2:GeneScript Multi prestained protein ladder

M3:Spectra BR Multicolor protein ladder

Protein expression Screening 3197_994_995_3225_3210

15ml medium, 0.5mM IPTG induce at RT for over night (around 14 hours), freeze and thraw on dry ice three times.

Lane	#	Name	Type	MW	Note	Pellet Type
M1	SeeBlue protein  ladder	4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191
1	3197	pT7A3-GST-BCMA	unbroken cell pellet supertant	35	Y	loose
2	994	pT7A3-GST-CD133-EC2	unbroken cell pellet supertant	53	Y	loose
3	995	pT7A3-GST-CD133-EC3	unbroken cell pellet supertant	50.1	Y	tight
4	3225	pT7A3-GST-MICA	unbroken cell pellet supertant	42.5	Y	tight
5	3210	pT7A30-Sumo-Ov-ASP1-BCMA	unbroken cell pellet supertant	36.7	?	loose
M2	GeneScript Broad Multi Color Pre-Stained Protein Standard	5kD, 15kD, 30kD(red), 35kD, 50kD(Green), 65kD, 95kD,130kD,175kD, and 270kD(purple).
6	3197		S		Y
7	994		S		Y
8	995		S		Y
9	3225		S		Y
10	3210		S		Y

Protein expression Screening 3218_824_999_944

15ml medium, 0.5mM IPTG induce at RT for over night (around 14 hours), freeze and thraw on dry ice three times.

Lane	#	Name	Type	MW	Note	Pellet Type
M1	SeeBlue	4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191
1	3218	pT7A30-ULP1	unbroken cell pellet		?	loose
2	3191	pT7A3-GST-Avitag	unbroken cell pellet	31	Y	loose
3	824	pET28b-VVTopo	unbroken cell pellet	39.9	Y	loose
4	999	pT7A30-SssI	unbroken cell pellet	46	N	tight
5	944	pET30b-pfu-exo-	unbroken cell pellet	91.1	N	loose
M3	Spectra BR protein ladder	5,10,25,35,40(red),50(green),70(brown),100,140(purple),260
6	3218		S
7	3191		S		Y
8	824		S		Y
9	999		S		N
10	944		S		Y