Protein Expression Clone Screening 2018

Protein Expression Clone Screening

Clone ID	Protein Name	Host	Condition	Pellet after lysis	Expression level	Solubility	Gel image	Date	By
1000	FnCpf1	Nico21(DE3)plysN	0.5mM IPTG, induce 4h, 37oC, OD value is around 0.8.	sticky		Y	Protein_QC_8_10_2018	8/10	yijia
942	MBP-VVN	Nico21(DE3)plysN	0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.	sticky		Y	Protein_QC_8_10_2018	8/10	yijia
938	PNGase F	Nico21(DE3)plysN	0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.	sticky		?	Protein_QC_8_10_2018	8/10	yijia
939	Tte-ExoIII	Nico21(DE3)plysN	0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.	sticky		Y	Protein_QC_8_10_2018	8/10	yijia
941	MBP-T7-Endo	Nico21(DE3)plysN	0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.	sticky		?	Protein_QC_8_10_2018	8/10	yijia
3197	GST-BCMA	Nico21(DE3)plysN	0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.	sticky		Y	Protein_QC_8_10_2018, Protein QC 8 14 2018	8/10	yijia
994	GST-CD133-EC2	Nico21(DE3)plysN	0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8.	sticky			Protein_QC_8_10_2018, Protein QC 8 14 2018	8/10	yijia
995	GST-CD133-EC3	Nico21(DE3)plysN	0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8.	not sticky			Protein_QC_8_10_2018, Protein QC 8 14 2018	8/10	yijia
3225	GST-MICA	Nico21(DE3)plysN	0.5mM IPTG, induce 4 h37oC, OD value is around 0.8.	not sticky		Y	Protein_QC_8_10_2018, Protein QC 8 14 2018	8/10	yijia
3210	Sumo-Ov-ASP1-BCMA	Nico21(DE3)plysN	0.5mM IPTG, induce 4 h 37oC, OD value is around 0.8.	sticky		Y	Protein_QC_8_10_2018, Protein QC 8 14 2018	8/10	yijia
3218	ULP1	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	not sticky		?	Protein QC 8 14 2018	8/14	yijia
3191	GST-Avitag	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	not sticky		Y	Protein QC 8 14 2018	8/14	yijia
824	VVTopo	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	sticky		Y	Protein QC 8 14 2018	8/14	yijia
999	SssI	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	sticky		N	Protein QC 8 14 2018	8/14	yijia
944	pfu-exo-	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	sticky		Y	Protein QC 8 14 2018	8/14	yijia
993		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
992		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
991		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
990		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
989		Nico21(DE3)plysN	1mMIPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
947		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
986		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
945		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 4 23 18	4/23	yijia
942		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 5 3 2018	5/3	yijia
944		Nico21(DE3)plysN	1mM IPTG,4oC,o/n	loose			Protein QC 5 3 2018	5/3	yijia
3122	CRM197	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	loose			Protein Expression 8 17 2018	8/17	yijia
3127	qBeta-qBeta	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	loose			Protein Expression 8 17 2018	8/17	yijia
984	SBP-SUMO-3C	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	loose			Protein Expression 8 17 2018	8/17	yijia
3248	T7-RNAP-P266L	Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	loose			Protein Expression 8 17 2018	8/17	yijia
129		Nico21(DE3)plysN	0.25mM IPTG,RT,o/n	loose			Protein Expression 8 17 2018	8/17	yijia