143 pT7A3-MBP-(Tev)-GST-(3C): Difference between revisions
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Latest revision as of 22:51, 18 November 2018
redo
10/27/2018 Redo PCR
need to check 143 clone #2.
sequencing: clone #2 no priming
11/5/2018, use new primers 143-F2
ATATATTCatg ACT AGT ggcaaaatcgaagaaggtaaac
143-MBP-R2
GAACCGGTGCCTCCACCGCCCTGGAAG TAAAGGTTTTC
digest MBP-GST with BspH1-Nde1, clone into NcoI-Nde1 digested vector 1851
11/8/2018
