Plasmid Miniprep By Isopropanol and PEG Precipitation

12/1/2018

Test CaCl2, MgCl2, CTAB for removing genomic DNA and RNA. Add MgCl2, CaCl2 to N3, final conc: 1.5 M in N3

CTAB add 20 ul 5% CTBA to 0.3 ml N3, Add 0.6 ml isopropanol to precipitate DNA . add EB to ethanol washed precipitate

water soluble fraction:

 

Water insoluble fraction, dissolve in 0.4 M NaCl:

http://www.excellgen.net/eln/images/7/74/CATB_Ca_Mg_on_Isopropanol_Plasmid2.JPG

12/2/2018

N3-CaCl2 on removing genomic DNA

Sample #	OD260	OD260 after PEG-NaCl ppt
1,	16 ng/ul
2	2695 ng/ul
3	35 ng/ul
4	1198  ng/ul
5	2605 ng/ul	251 ng/ul
6	1131 ng/ul	117 ng/ul
7, 2203	22 ng/ul
8, 2203	1494 ng/ul
9, 2203		121 ng/ul