165 pT7A3-MBP-(TEV): Difference between revisions

From ELN

Jump to navigation Jump to search

Latest revision as of 14:24, 2 January 2019

Redo

Clone annealled oligos into Xba1-Nco1 digested  2369 pT7A3-lc-MBP-(TEV)
2094-T
CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAT
    TTTATTAAAACAAATTGAAATTCTTCCTCTATATAGTAC
2094-B
CATGATATATCTCCTTCTTAAAGTTAAACAAAATTATTT

2094 should not grow on Kan plate, but there are colonies. so it is contaminated clone,

1/2/2019, digestion NcoI+ Xho1

http://www.excellgen.net/eln/images/8/8e/165_168_digestion.JPG

Retrieved from "https://eln.excellgen.com/index.php?title=165_pT7A3-MBP-(TEV)&oldid=19785"

Cloning