Commercial Enzymes

Excellgen Cat #	Clone #	Size	Price	Name	Supplier Cat #	Supplier	Lot	Concentration	Size	Storage Buffer	Price
EG-150		100units, 10U/ul, 10ul
EG-151		2,000 U/ml,50ul, 100 units	$85	PolyU polymerase,   Schizosaccharomyces pombe	M0337S	NEB		0.34 mg/ml	2000U/ml, 30 ul, 60 units		$85
EG-1057	1942	100,000 U/ml 100U/ul 1ug/ul, 100 ul, 100ug	$350	phi29 (φ29) DNA Polymerase	EP0094	Thermofisher		0.1ug/ul, 10u/ul,	5000U 500 ul --> 50 ug 50 ul of 1 mg/ml stock + 450 ul buffer	50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycero	$826
				E coli polyA polymeras	M0276S	NEB			20ul, 7.43 mg/ml by reading but has 0.1% Triton X-100
				T4 DNA ligase , 400units/ul	M0202L	NEB			1.1mg/ml 0.76mg/ml (1271711)
				T4 DNA ligase. 2U/ul	F93301	Lucigen			2.7mg/ml
				T4 DNA ligase. 2U/ul	46-0117	invitrogen			0.61mg/ml
				T4 DNA ligase. 5U/ul	K-1232	Thermofisher	00680547		1.03mg/ml
				Tag Ligase, 40,000U/ml	M0208S	NEB			0.3/mg/ml	50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 µg/ml BSA and 50% glycerol.
				Bst polymerase, large fragment	M0275S	NEB		8000U/ml	2.32mg/ml not accurate because Tx-100	storage buffer has Triton X-100 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% Triton® X-100 pH 7.1 @ 25°C based on NEB's specific Activity: 120,000 units/mg,  100U/ul = 20,000U/200ul, 90% purity, then need 200 ug protein in 200 ul., take 10 ul of 29mg/ml-->200 ul storage buffer, protein conc is 1.7 mg/ml
				T3	M0317S	NEB		3000U/ul	2mg/ml
EG-160				T4 PDG	M0308S	NEB		10U/ul	3.93mg/ml	storage buffer has 0.15% Triton X-100, do not know exact protein conc
EG-1005				APE 1	M0282S	NEB		10U/ul	1.24