2644

both 2642 #2, #3 no priming

Expression screening using 10 ul magnetic beads from expedeon Amintra® Nickel Magnetic Affinity Resin

Lane	ID	Name	Host	MW	Induction	Note	Expression
1	375	pET28-Cre	Nico21(DE3)pLysN,	10.30 / 42.8	1 mM IPTG, 18 oC	grown on LB/KC, most cells are lysed	y
2	375	pET28-Cre	Rosetta(DE3)	10.30 / 42.8	1 mM IPTG	looks like contaminated, can not lyse cells	trace
3	375	pET28-Cre	Nico21(DE3)pLysN	10.30 / 42.8	1 mM IPTG	grown on LB/K	y
4	2603	pL6H-sso7d-SD-Taq	Nico21(DE3)pLysN	6.10 / 104.7	1 mM IPTG		y
5	2642	pET29b-Flp	Nico21(DE3)pLysN	9.76 / 52.5	1 mM IPTG	most cells are lysed	n
M			pageruler plus
6	2643	pET29b-Int-C3	Nico21(DE3)pLysN	9.69 / 41.4	1 mM IPTG		maybe
7	2643	pET29b-Int-C3	Nico21(DE3)pLysN	9.69 / 41.4	1 mM IPTG		maybe
8	2644	pET29b-scIHF2	Nico21(DE3)pLysN	9.60 / 22.8	1 mM IPTG		?
9	1010	Cre	Nico21(DE3)pLysN			Lot 174-1010
10	1186	MBP or MBP-Xis

1/2/2020, small scale expression screening using magnetic beads, 15 ml culture medium, induction at 37 oC

Lane	ID	Sample	Competent Cells	Induction
1	2642	Soluble	BL21-AI	0.2% arabinose 4 hours
2	2642	Inclusion body	BL21-AI	0.2% arabinose 4 hours
3	2642	Soluble	Nico21(DE3)	0.5M IPTG 4 hours
4	2642	Inclusion body	Nico21(DE3)	0.5M IPTG 4 hours
5	2643	soluble	Nico21(DE3)	0.5M IPTG 4 hours
6	2644	soluble	Nico21(DE3)	0.5M IPTG 4 hours
M	PageRuler Plus