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Latest revision as of 09:14, 28 October 2011
Cloning of Turo-TEV
Purpose: to clone Turo-TEV
Date: 2011-10-14
Notebook:Liang Huo
Page:
Clone ID:203
Experimenters: Liang Huo
Results:
20 oligo first round Two PCR reactions, then Using outside primers second round PCR, fragement ~800bp
EcorI+Sal1 digest pET28b and PCR fragement, gel purified, ligation O.N at 16 degree.
8 clones screening( after ECoRI and Sal1 digested):
Picked clone 3 for sequencing, using T7 term as seq primer