Transfection reagents comparison 13: Difference between revisions
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Latest revision as of 10:43, 29 October 2011
Make new transfection reagent (TR).
3 ul TR + 50ul DMEM without serum---->Mix A
1 ug DNA + 50ul DMEM without serum---->Mix B
Mix A + Mix B, incubate 20min at room temperature
Add 100 ul mixture to transfect 293T cells in 12 well-plate.
48 hours after transfection, picture taken on 10-26-2011
48 hours after transfection, picture taken on 10-26-2011
72 hours after transfection, picture taken on 10-28-2011
10-29-2011