Proteins in Freezer: Difference between revisions

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Latest revision as of 19:24, 2 December 2021

Box P1

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified by


Taq

1.19mg/ml
3




Taq


1.42 mg/ml
3




Taq


4.21 mg/ml
4




Taq


0.48 mg/ml
2


67-2038
Taq


1.5mg/ml
2


20130724
KOD


10mg/ml 0.5 ml 2




Phusion 6-27-12 CM 4.4 mg/ml
16




Phusion


17.5 mg/ml






Phusion
5/18/18 QC





20140203
Kfu

1mg/ml






Kfu


1.26mg/ml





2013/8/14
1498 Taq DNA Ligase  2013/8/14
 pT7A3-TaqDNAligase
9mg/ml




162-1483
T4 DNA ligase 6/26/19

2.8 mg/ml





Box P2

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By

1747 MMLV 2013/10/11 pT7A30-MMLV 6mg/ml
2


81-2264
Gst-Polymerase

29.6 mg/ml
6



1811 V-Pol 2013/10/15
44.6 mg/ml
2




V-Pol 2013/10/15








2462 TN5 Transposase

8.86 mg/ml

5




TN5 Transposase



10 ml 1




LacI-SV40 2014/2/11
4.75 mg/ml





1797 3C-MBP 2013/8/14 pCold-6His-3C 2.6mg/ml
6


1402/11 1941


0.45ml






FN3K 20131014
0.93mg/ml




168-2565
2565 Thermostable MMLV 7/18/19
0.46mg/ml 6ml 3 vials




MMLV EG-1029







Box P3

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
82-2392
UVSX

18.6 mg/ml 500ul 24


72-2196

2196

EG-1063 PBCV-1 DNA Ligase Chorella virus DNA Ligase
10/8/14
8mg/ml

14


20130806
MBP-(3C-TEV)-PKARIIB




6


219-1593

E coli Endonuclease IV (Endo IV), NfoEG-160
6/1/21
1mg/ml
5000U 7

Paul

Box P4

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
20121223
T4 DNA ligase

12mg/ml
3




T4 DNA ligase


41/mg/ml
2


20130204
T3 DNA ligase




15


20121207 1483 T7 DNA ligase


4.5mg/ml
15

























































Box P5

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
58-2107 EG-25 Taq Muts

4mg/ml
38


58-2107
EG-41 Taq Muts


4mg/ml 100ug



207-1078
EG-39 IHF 11/23/2020
3mg/ml 90 ul



206-1074
EG-39
IHF 11/23/2020
2mg/ml
90 ul




208-1203
EG-39 GST-IHF 11/23/2020

3mg/ml
90 ul
















































90 ul

Box P6

[edit]
Lot ID Name Cat Date Description Concentration Size Quantity Position QC Purified By
  His-MBP-Muts EG-197
10/28/11 all tubes 50% Gly 1.5mg/ml 0.5mg 41
0117 EG-9 Taq-Muts-protein
 12mg/ml 500ug 11


























Box P7

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
  Taq-Muts 20%Gly 1mg/ml 50ug 20
88-212 212 Tth-Muts 7/24/15 2.52mg/ml 48


158-212 212 Tth-Muts 3/8/19 P7R2.4 is good, red cap
2.38 50ug 48 +1 .5ml


220-129
129
Tth-Muts
Jul 03 2021
Ni--> QFF QFF: large column 2.5 cmX10 cm QFF, dilute Buffer B ( 500 mM NaCl, 25 mM Tris-HCl, pH 8.0, 150 mM Imadazole) eluante with 2 vol of dilution buffer (25 mM Tris-HCl, pH8.0, 50 mM NaCl), load to the column,  wash column with dilution buffer, then elute with 1 M NaCl in Dilution buffer. Small column (1.6 cmX10 cm), dilute  eluant from the large QFF column further with 1 vol of dilution buffer, load to small column,  wash column with dilution buffer, then elute with 1 M NaCl in Dilution buffer. 

Large column:  2mg/ml, 6 X 1 ml, total 12 mg 

Small column: 2mg/ml, 8.5 ml, total 17 mg






Box P8

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
  Taq 04/21/2016 79
 














Box P9

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
  Taq-Muts green cap 8.6mg/ml 0.5mg 17
no name, probably is Tth Muts protein6Xhis-Tth-Muts  7.4mg/ml 500ug 32













Box P10

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
89-1529 Ecoli Rec A 7/27/15 1.6mg/ml 1mg 16
89-1529 Ecoli Rec A 7/27/15  1.6mg/ml 200ug 18



































Box P11

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
 20130224 GST-3C 6.78mg/ml 13
TEV 02/03/11  1mg.ml 4




GST-TEV



2


12152110 RP-8 Turbo-TEV protesse

10U/ul 1mg/ml
38














































Box P12

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
  Taq Ligase 2mg/ml,20000 unit
Tag Ligase  3.77mg/ml



























































Box P13

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified by


unknown, maybe transfection reagent







Box P14

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
57-1005,155-1005 EG-1068 Tat-GFP 1mg 17
56-375 RP-7 Cre recombinase 2mg/ml 1mg 9


20130826 RP-7 Cre recombinase

5mg/ml 1mg 4


121913 EG-1001 HNC-Cre recombinase

2mg/ml 1mg 11



RP-18 HTN-GFP

1mg/ml
1


5414 RP-22 FLP recombinase

1mg/ml 0.5mg














Box P15

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
 63-2288 2288 SUMO protein SMT3 4mg/ml 0.5mg 3
2289 SMT3 1mg/ml 3


70-212 212 Tth-Muts

5.8mg/ml
6



211 MBP-Taq-Muts

14mg/ml
2




MBP--Taq-Muts

3.5mg/ml
1




429--pHisMBP-TVMV,

1512-->Phi29 DNA Polymerase,???



1.47mg/ml
3


1-165
M1wt



2













Box P16

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
  1737 TRX NN 4mg/ml 9
65-1737 1737 TRX 13mg/ml 0.5mg 4



1736 NusA

4mg/ml
13



1736 NusA

4.5mg/ml
7



1736 NusA

3mg/ml
15


170-2616
2616 CYFRA 21-1


2mg/ml 30 mg 11


171-1185
1185 MBP

2mg/ml 14 mg 4


Box P17

[edit]
Lot ID Name Date Description Concentration Size Quantity Position QC Purified By
 182-2565 2565 Thermostable reverse transcriptases MMLV 6/22/2020

13mg/ml white cap

1.3 mg/ml blue cap

21

10

184-2779
2779 Polynucleotide phosphorylase
7/1/2020

7mg/ml.

1 mg/ml

3

1





216-2932

Cas13A (sumo digested)
2/8/2021
16.72 mg/ml
4



221-2565
MMLV Thermostable, EG-1061 12/2/2021
5 mg/ml 15 ml



217-2932

Cas13A (not digested)
2/16/2021

5.13mg/ml 

2.5 mg/ml

11

1