Sequence Protocol: Difference between revisions
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Latest revision as of 11:04, 15 July 2022
Psomagen Sanger sequencing protocol:
Plasmid and primer separately: 150 ng/ul plasmid x 10 ul, 10 uM primer x 5 ul;
Premix: 100 ng/ul x 5 ul plamid containing 5 pmol primer (total amount).