Transfection reagents freeze-thaw 100 times: Difference between revisions
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Latest revision as of 12:21, 26 November 2011
Make transfection reagent (TR) to freeze-thaw 100 times.
3 ul TR + 50ul DMEM without serum---->Mix A
1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B
Mix A + Mix B, incubate 20min at room temperature
Add 100 ul mixture to transfect 293T cells in 12 well-plate.
0 is control.
48 hours after transfection
2011-11-24 (3 days after transfection)
--Xiaojing Wang 12:04, 23 November 2011 (EST)