Transfection reagents comparison 14: Difference between revisions
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Latest revision as of 10:54, 28 November 2011
- Make 7 different transfection reagents (TR).
3 ul TR + 50ul DMEM without serum---->Mix A
1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B
Mix A + Mix B, incubate 20min at room temperature
Add 100 ul mixture to transfect 293T cells in 12 well-plate.
1-1,1-2,2-1,2-2,3-1 are EG-1031, lot 20111107.
4-1,4-2 are lot 20110930.
48 hours after transfection, picture taken on 11-4-2011
48 hours after transfection, picture was taken on 11-7-2011.
- Make 4 different transfection reagents (TR).
3 ul TR + 50ul DMEM without serum---->Mix A
1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B
Mix A + Mix B, incubate 20min at room temperature
Add 100 ul mixture to transfect 293T cells in 12 well-plate.
1 is EG-1086, lot 20110714.
2 is EG-1074, lot 20110803 (Gene Expresso 8000).
3 is EG-1031, lot 20111107.
4 is EG-1086T, lot 20111110.
48 hours after transfection, picture was taken on 11-11-2011.