457-PET28b-CymR: Difference between revisions
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Latest revision as of 17:11, 8 December 2011
Cloning of 457-PET28b-CymR
Purpose: gene synthesis to cloning of 457-PET28b-CymR
Date: 2011-11-28----
Notebook:Lu yingying
Page:
Clone ID:457
Experimenters: lu yingying
Results
Gene synthesis
template: CymR1 to CymR18 mixture
CymR 1 and CymR18 as primers
digest:BamH1+ Xho1
band 1 show insert ,band 2 show PET28b vector
Date: 2011-12-6----
Notebook:Lu yingying
Page:
Clone ID:457
Experimenters: lu yingying
Results
Gene synthesis
template: CymR1 to CymR18 mixture
CymR 1 and CymR18 as primers
digest:BamH1+ Xho1
band 1 show insert ,band 2 show PET28b vector
digest detect: BamH1+ Xho1