422-pET30b-sso7d-DeepVent: Difference between revisions
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Latest revision as of 14:00, 9 December 2011
Cloning of pET30b-sso7d-DeepVent
Purpose: to clonepET30b-sso7d-DeepVent by PCR
Date: 2011-12-8
Notebook:Lu yingying
Page:
Clone ID:422
Experimenters: lu yingying
Results
PCR using NEB720 Deep Vent (385) as template
to clone into pET30b-sso7d-NK 369
DVent-Nco1-F
atcgaa CC ATGGtacttgacgctgactac
DVent-Sal1-R
(ggcttaacatcaagaagaag GTCGAC atcaga)
tctgatGTCGACcttcttcttgatgttaagcc
--Yingying.lu 13:00, 9 December 2011 (EST)