414-pAd5-CMV-Cre: Difference between revisions
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Latest revision as of 15:56, 29 December 2011
Cloning of pAd5-CMV-Cre
Purpose: to clone pAd5-CMV-Cre by subclone from241 pLentiGP-Cre to 280 pAd5-CMV-MCS
Date: 2011-9-29
Notebook:Lu yingying
Page:
Clone ID:414
Experimenters: lu yingying
Results
bands 3 show cre insert ,bands 4 show pAd5 vector
Ligation at RT for 2h
digest detect:EcoR1+Xho1
Date: 2011-10-25
Notebook:Lu yingying
Page:
Clone ID:414
Experimenters: lu yingying
Results
band 1,2 show subclone digest result:EcoR1+Xho1
241 pLentiGP-Cre to 280 pAd5-CMV-MCS, band 1 show 241 digest result,band 2 show 280 digest result
band 414,1-6 show digest detect result:EcoR1+Xho1
Cloning of pAd5-CMV-Cre
[edit]Purpose: Cloning of pAd5-CMV-Cre from241 pLentiGP-Cre to 280 pAd5-CMV-MCS
Date: 2011-12-14---
Notebook:
Page:
Clone ID: 414
Experimenters: yingying
Results
[edit]Subcloning
[edit]Date: 2011-12-14---
| 414 |
band1 |
band2 |
| 241 pLentiGP-Cre |
280 pAd5-CMV-MCS | |
| digest |
EcoR1+Xho1 |
EcoR1+Xho1 |
Colony Screening
[edit]Date: 2011-12-16
Make miniprep and digest with EcoR1+Xho1
lanes 1,2,3,5,6,7 show right, need to sequence
Result intepretation/Summary
[edit]- Sequencing Result:414-6 right
Conclusion
[edit]414-6 right
==
--Yingying.lu 08:47, 19 December 2011 (EST)