459-PCMVK-Gluc-PKA-Alpha: Difference between revisions
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Latest revision as of 16:28, 3 January 2012
Cloning of 459-pAAV-CMV-RFP
Purpose: to PCMVK-Gluc-PKA-Alpha by PCR
Date: 2011-12-6----
Notebook:Lu yingying
Page:
Clone ID:459
Experimenters: lu yingying
Results
PCR using pLentiGP-Gluc-PKAalpha 300 as template and the following primers
Gluc-Xba1-F
atcga TCTAGA CCGGCCACCATGGGAGTCAAAG
PKA-6His-BamH1-R
(gcaaggagttttctgagttt CTC GAG CAT CAC CAT CAC CAT CAC TAA GGA TCC aaatag)
Cut PCR product with Xba1 + BamH1, clone into pCMVK-StrepII 193 (Xba1 + BamH1)
digest detect: Xba1 + BamH1
--Yingying.lu 13:08, 12 December 2011 (EST)
Date: 2011-12-20----
Notebook:Lu yingying
Page:
Clone ID:459
Experimenters: lu yingying
Results
PCR using pLentiGP-Gluc-PKAalpha 300 as template and the following primers
Gluc-Xba1-F
atcga TCTAGA CCGGCCACCATGGGAGTCAAAG
PKA-6His-BamH1-R
(gcaaggagttttctgagttt CTC GAG CAT CAC CAT CAC CAT CAC TAA GGA TCC aaatag)
Cut PCR product with Xba1 + BamH1, clone into pCMVK-StrepII 193 (Xba1 + BamH1)
digest detect: Xba1 + BamH1