528-pLVX-IRES-EGFP: Difference between revisions
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Latest revision as of 13:00, 9 January 2012
Cloning of 528-pLVX-IRES-EGFP
[edit]Purpose: Cloning of pLVX-IRES-EGFP by PCR
Date: 2011-12-30---
Notebook:
Page:
Clone ID:528
Experimenters: yingying
Results
[edit]
Primers
IRES-Xho1-F
Xho1
atcgca ctcgag TACGTAAATTCCGCCCCTCTC
IRES-Mlu1-R
(CATGGACGAGCTGTACAAGTAA ACGCGT acgcatc)
gatgcgtACGCGTTTACTTGTACAGCTCGTCCATG
PCR with IRES-Xho1-F + IRES-Mlu1-R using pAd5-CMV-2-IRES-GFP 242 as template
| band 1 |
band 2 | |
| PCR product |
pLVXPlus2-IRES-mCherry, 174 | |
| digest enzyme |
Xho1+Mlu1 | |
Subcloning
[edit]Date:
Colony Screening
[edit]Date: 2012-1-6
Make miniprep and digest with Xho1+Mlu1
transfection result: 528-1
Result intepretation/Summary
[edit]- Sequencing Result:
Conclusion
[edit]Restriction digestion
[edit]
--Yingying.lu 09:21, 3 January 2012 (EST)