501-pET30b-MMLV: Difference between revisions

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Latest revision as of 15:05, 12 January 2012

== Cloning of '501-pET30b-MMLV'

Purpose: Subcloning of MMLV-C from pET30b-MMLV-C 500 pET30b-MMLV-N 477

Date: 2012-1-9----

Notebook:

Page:

Clone ID:501

Experimenters: yingying

Results

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Subcloning

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Date: 2012-1-9



band 1
band2

pET30b-MMLV-C 500
pET30b-MMLV-N 477
digest enzyme
Nco1 + Xho1
Nco1 + Xho1







Colony Screening

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Date:2012-1-11

Make miniprep and digest with Nco1 + Xho1




Result intepretation/Summary

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Conclusion

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-Yingying.lu 09:23, 3 January 2012 (EST)