468-pAd5.CMV-Cre-EGFP: Difference between revisions

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Latest revision as of 16:14, 23 January 2012

Cloning of 468-pAd5.CMV-Cre-EGFP

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Purpose: Cloning of 468-pAd5.CMV-Cre-EGFP by PCR

Date: 2012-1-18--

Notebook:

Page:

Clone ID:468

Experimenters: yingying

Results

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AAV8.2 (not 2) - Cre-EGFP as template

Cre-EcoR1-F2+EGFP-Xho1-R


band 1 insert


band 1 vector: pAd5-CMV-MCS 280

EcoR1+Xho1

Subcloning

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Date:





Colony Screening

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Date: 2012-1-23

Make miniprep and digest with  EcoR1+Xho1



Result intepretation/Summary

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Conclusion

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