257-pET28b-HTNCre-ssfGFP: Difference between revisions
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Latest revision as of 11:18, 24 January 2012
Cloning of 257-pET28b-HTNCre-ssfGFP
[edit]Purpose: Cloning of 257-pET28b-HTNCre-ssfGFP by PCR
Date: 2012-1-17--
Notebook:
Page:
Clone ID:257
Experimenters: yingying
Results
[edit]
pTriEx-HTNC 298 as template
6XHis-NcoI-F + Cre-Nhe1-R
| band 1 |
band 2 | |
| PCR product |
pET28b-ssfGFP 169 | |
| digest enzymes |
Nco1+ Nhe1 | |
Subcloning
[edit]Date
Colony Screening
[edit]Date: 2012-1-
Make miniprep and digest with
Result intepretation/Summary
[edit]Conclusion
[edit]
[edit]
Cloning of pET28b-HTNCre-ssfGFP
[edit]Purpose: Cloning of pET28b-HTNCre-ssfGFP
Date: 2012-1-20
Notebook:
Page:
Clone ID:257
Experimenters: yingying
Results
[edit]
| band 3 |
band 4 | |
| PCR product |
pET28b-ssfGFP 169 | |
| digest enzymes |
Nco1+ Nhe1 | |
Subcloning
[edit]
Colony Screening
[edit]Date: 2012-1-23
Make miniprep and digest with Nco1+ Nhe1
clone 1,2,3,4,5 may right
Result intepretation/Summary
[edit]Conclusion
[edit]
[edit]