535-pAAV-CMV-DIO-sfGFP

Cloning of 535-pAAV-CMV-DIO-sfGFP

Purpose: Cloning of 535-pAAV-CMV-DIO-sfGFP by genesythesis

Date: 2012-1-24--

Notebook:

Page:

Clone ID:535

Experimenters: yingying

Results

Cloning

Date: 2012-1-24

gene synthesis,

PCR1:DIO-sfGFP1 to DIO-sfGFP12

PCR2:DIO-sfGFP13 to DIO-sfGFP26

PCR: PCR1+PCR2 as template,

primer:sfGFP1+DIO-sfGFP26

	band 1	band 2
	PCR product	254 pAAV.CMV-MCS
digestion enzymes	EcoR1 + BamH1

Colony Screening

Date: 2012-1-30

Make miniprep and digest with  EcoR1 + BamH1

535-1,2,3,4,5,6 may right ,need to sequence

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