542-pTracer- BcCD: Difference between revisions
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Latest revision as of 15:21, 30 January 2012
Cloning of 542-pTracer- BcCD
[edit]Purpose: Cloning of 542-pTracer- BcCD by PCR
Date: 2012-1-26--
Notebook:003719
Page:
Clone ID:542
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-1-26
PCR using BcCD 539 as template
Primer-F pBcCD-F, AGGGAGACCCAAGCTGGCTAGC gcc acc ATG TCT AAT AAT GCA CTC C
Primer-R pBcCD-R, ACGGGCCCTCTAGACTCGAGCGGCCGCTCACCTTTTATAATCAATTGC
| band 1 |
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| PCR insert |
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| digest enzymes |
Nhe1 + Not1 |
| band | |
| pTracer.CMV/Bsd 538 | |
| digest enzymes |
Nhe1 + Not1 |
Colony Screening
[edit]Date: 2012-1-30
Make miniprep and digest with Nhe1 + Not1
clone 1,2,3,4,5 may be right, need to sequence
Result intepretation/Summary
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Conclusion
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