532-PET28b-mIL10: Difference between revisions
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Latest revision as of 14:29, 6 February 2012
Cloning of PET28b-mIL10
[edit]Purpose: Subcloning of PET28b-mIL10 from 529 pLVX-mIL10-IRES-EGFP to PET28b
Date: 2012-2-1
Notebook:
Page:
Clone ID:532
Experimenters: yingying
Results
[edit]Subcloning
[edit]Date: 2012-2-1
529-3 digested band:BamH1 + Xho1 as 532 insert
band 2: digested vector: BamH1 + Xho1
Colony Screening
[edit]Date: 2012-2-1
Make miniprep and digest with BamH1 + Xho1
clone 1,5 may be right
Result intepretation/Summary
[edit]Conclusion
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