323-pLenti-EF1-MCS: Difference between revisions
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Latest revision as of 15:56, 10 February 2012
Cloning of 323-pLenti-EF1-MCS
[edit]Purpose: Cloning of 323-pLenti-EF1-MCS
Date: 2011-12-28
Notebook:
Page:
Clone ID:323
Experimenters: yingying
Results
[edit]
template: pPuroGreen 178
Primer-F EF1-NdeI-F agct GCTAGC c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag
| band 3 |
band 4 | |
| PCR product |
pLenti-Plus (152) | |
| digest enzyme |
| |
Subcloning
[edit]Date:
Colony Screening
[edit]Date: 2012-1-3
Make miniprep and digest with Nde1 + BamH1
Result intepretation/Summary
Conclusion
[edit]
--Yingying.lu 15:05, 29 December 2011 (EST)
Cloning of 323-pLenti-EF1-MCS
[edit]Purpose: Cloning of 323-pLenti-EF1-MCS
Date: 2012-1-4
Notebook:
Page:
Clone ID:323
Experimenters: yingying
Results
[edit]
template: pPuroGreen 178
Primer-F EF1-NdeI-F agct GCTAGC c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag
| band 1 |
band 2 | |
| PCR product |
pLenti-Plus (152) | |
| digest enzyme |
| |
Subcloning
[edit]Date:
Colony Screening
[edit]Date: 2012-1-6
Make miniprep and digest with Nde1 + BamH1
Result intepretation/Summary
Conclusion
[edit]
--Yingying.lu 15:05, 29 December 2011 (EST)
Cloning of 323-pLenti-EF1-MCS
[edit]Purpose: Cloning of 323-pLenti-EF1-MCS by PCR
Date: 2012-1-26--
Notebook:
Page:
Clone ID:323
Experimenters: yingying
Results
[edit]
cloning
[edit]Date: 2012-1-26
Primer-F EF1-NdeI-F agct CATATG c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag
| band 3 |
band 4 | |
| PCR product |
152 pLenti-plus | |
| digestion enzymes |
Nde1 + BamH1 | |
Colony Screening
[edit]Date: 2012-1-30
Make miniprep and digest with Nde1 + BamH1
clone 1,2,5 may be right, need to sequence
Result intepretation/Summary
[edit]- sequencing :no priming ,need to clone again
Conclusion
[edit]
Cloning of 323-pLenti-EF1-MCS
[edit]Purpose: Cloning of 323-pLenti-EF1-MCS by PCR
Date: 2012-2-7--2012-2-10
Notebook:003732
Page:
Clone ID:323
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-7
template:178- pGreenPuro(EF1)
Primer-F EF1-NdeI-F agct CATATG c gtgaggctcc ggtgcccg
Primer-R EF1-BamH1-R (cttccatttcaggtgtcgtga GGATCC atcg) cgatGGATCCtcacgacacctgaaatggaag
| band 1 |
band 2 | |
| PCR product |
152 pLenti-plus | |
| digest enzymes |
Nde1 + BamH1 | |
Colony Screening
[edit]Date: 2012-2-10
Make miniprep and digest with Nde1 + BamH1
no insert
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
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